human colon cancer cell lines 293t  (China Center for Type Culture Collection)

Journal: Iranian Journal of Pharmaceutical Research : IJPR

Article Title: Gamabufotalin Suppresses Colorectal Cancer Growth via Oxidative Stress-Induced Apoptosis and DNA Synthesis Inhibition

doi: 10.5812/ijpr-169859

Figure Lengend Snippet: In vitro experiments demonstrated that gamabufotalin (GA) has a dose-dependent inhibitory effect on colorectal cancer (CRC) cells. A, the chemical formula of GA; B, SW480, HCT-116, HT-29, NCM460, and 293T cells were treated with 0-20000 nM GA for 48 hours, and viability was initially determined using the CCK-8 kit. Each concentration point was repeated six times (n = 6). C, CRC SW480, HCT-116, and HT-29 cells were co-incubated with GA at concentrations ranging from 0 to 200 nM for 48 hours, and the relative cell viability of each experimental group was evaluated using the CCK-8 kit. D, the cell viability of SW480, HCT-116, HT-29, NCM460, and 293T cells was measured using the CCK-8 kit at a GA concentration of 50 nM. E, the cell viability of CRC SW480 and HCT-116 cells was measured under a serum concentration of 1% in the culture condition. F, SW480 cells were treated with GA for 48 hours, and the morphological changes in SW480 cells caused by GA were photographed using an inverted microscope. G, SW480 cells were treated with different concentrations of GA for 24 hours, and the width of the scratch was measured, and the cell scratch closure rate was calculated. Post hoc analysis using LSD was performed after one-way ANOVA to evaluate significance, ** P < 0.01.

Article Snippet: SW480, HCT-116, NCM460, and 293T human colon cancer cell lines were procured from the American Type Culture Collection (ATCC).

Techniques: In Vitro, CCK-8 Assay, Concentration Assay, Incubation, Inverted Microscopy

Journal: Molecular Medicine Reports

Article Title: MicroRNA-142-3p suppresses cell proliferation, invasion and epithelial-to-mesenchymal transition via RAC1-ERK1/2 signaling in colorectal cancer

doi: 10.3892/mmr.2021.12207

Figure Lengend Snippet: miR-142-3p regulates RAC1 expression. (A) Binding sites of miR-142-3p in the 3′-UTR of RAC1 (red text). (B) Luciferase activity after co-transfection of miR-142-3p mimics and WT-RAC1 or MUT-RAC1. (C) Expression of miR-142-3p by quantitative PCR after transfection with miR-142-3p. (D) Expression of RAC1 determined by western blotting after transfection with miR-142-3p. *P<0.05; **P<0.01. miR, microRNA; WT (wild-type SW620 cells transfected with WT- RAC1 ); MUT, mutant (SW620 cells transfected with MUT- RAC1 ); PC, positive control cells; NC, negative control cells; RAC1, RAC family small GTPase 1; UTR, untranslated region.

Article Snippet: The human colon cancer cell lines, SW620 and 293T, were purchased from the China Center for Type Culture Collection.

Techniques: Expressing, Binding Assay, Luciferase, Activity Assay, Cotransfection, Real-time Polymerase Chain Reaction, Transfection, Western Blot, Mutagenesis, Positive Control, Negative Control

Journal: Molecular Medicine Reports

Article Title: MicroRNA-142-3p suppresses cell proliferation, invasion and epithelial-to-mesenchymal transition via RAC1-ERK1/2 signaling in colorectal cancer

doi: 10.3892/mmr.2021.12207

Figure Lengend Snippet: Effect of miR-142-3p on the viability of colon cancer SW620 cells. (A) Cell Counting Kit-8 assays were used to measure cell proliferation in SW620 cells transfected with miR-142-3p mimics or RAC1 inhibitor. (B) Representative images of colony-formation assays of SW620 cells (magnification, ×40). (C) Average number of colonies in SW620 cells transfected with miR-142-3p mimics or RAC1 inhibitor. (D and F) Transwell and (E and G) Matrigel invasion assay of SW620 cells transfected with miR-142-3p mimics or RAC1 inhibitor (magnification, ×100). *P<0.05; **P<0.01. miR, microRNA; RAC1, RAC family small GTPase 1; KD, miR-142-3p mimic cells; NC, miR-142-3p mimic negative control cells; CON, control cells without any treatment; NSC, RAC1 inhibitor NSC23766.

Article Snippet: The human colon cancer cell lines, SW620 and 293T, were purchased from the China Center for Type Culture Collection.

Techniques: Cell Counting, Transfection, Invasion Assay, Negative Control, Control

Journal: Molecular Medicine Reports

Article Title: MicroRNA-142-3p suppresses cell proliferation, invasion and epithelial-to-mesenchymal transition via RAC1-ERK1/2 signaling in colorectal cancer

doi: 10.3892/mmr.2021.12207

Figure Lengend Snippet: miR-142-3p blocks EMT by regulating RAC1/ERK1/2 signaling in colon cancer SW620 cells. (A) Representative results of western blot analysis of EMT markers and signaling proteins after transfection with miR-142-3p mimics and treatment with RAC1 inhibitor. (B) Relative expression of EMT markers and signaling protein after transfection of miR-142-3p mimics and treatment with RAC1 inhibitor. *P<0.05; **P<0.01. miR, microRNA; EMT, epithelial-to-mesenchymal transition; RAC1, RAC family small GTPase 1; KD, miR-142-3p mimic cells; NC, miR-142-3p mimic negative control cells; CON, control cells without any treatment; NSC, RAC1 inhibitor NSC23766; MMP, matrix metalloproteinase; p-, phosphorylated-.

Article Snippet: The human colon cancer cell lines, SW620 and 293T, were purchased from the China Center for Type Culture Collection.

Techniques: Western Blot, Transfection, Expressing, Negative Control, Control